rsref is a scripted modular workflow for the refinement of atomic models against high-resolution cryo-EM density maps. It also features graphical output for model validation. It is a not a standalone refinement software, but rather provides a set of tools for model and/or map manipulation, refinement protocols, the analysis of the refinement cycles and validation of the resulting coordinate models.

The development of rsref started in the Sachse lab at EMBL Heidelberg and is now continued in our lab at the Kavli Institute.

Atomic coordinate refinement is not a trivial task at low resolution (3.5 - 6.0 A). The scripts and programs provided here are intended to help make decisions and automate the process, in particular for maps with a substantial degree of resolution variation.

rsref is still under development and not currently officially released. If you are fine with limited support, please see the Wiki pages for dowload and usage instructions and tutorial.

If rsref is useful for your research please acknowledge its use.

Hoffmann, Jakobi et al., Nature 528:231-236 (2015)     doi: 10.1038/nature16143
Hoffmann, Jakobi et al., FEBS J 283:2811-2819 (2015)     doi: 10.1111/febs.13732